; pushoneshotzs ; ; Zangger-Sterk pure shift DOSYsequence ; uses push1dzs and oneshot sequence ; Uses pulse order: broadband then selective (unpublished) ; J is refocussed at centre of chunk ; ; Ralph Adams, Juan Aguilar, Robert Evans, Mathias Nilsson and Gareth Morris ; University of Manchester ; Release 1.0c (18Oct2011) ; Avance III Version ; ; Work published using this sequence should cite: ; J.A. Aguilar, S. Faulkner, M. Nilsson and G.A. Morris, Angew. Chem. Int. Ed.; 2010, 49, 3901-3903. ; ; A one-shot sequence for high-resolution diffusion-ordered spectroscopy ; Michelle D. Pelta, Gareth A. Morris, Marc J. Stchedroff, Stephen J. Hammond ; Magnetic Resonance in Chemistry. Volume 40 Issue 13, Pages S147 - S152 ; doi: 10.1002/mrc.1107 ; ; ; Other relevant papers that could be of use include: ; G.A. Morris, J.A. Aguilar, R. Evans, S. Haiber and M. Nilsson, J. Am. Chem. Soc.; 2010, 132, 12770 - 12772. ; M. Nilsson and G.A. Morris, Chem. Commun.; 2007, 9, 933 - 935. ; ; Data can be reconstructed using the 'pshift' macro. This produces a set of ; attenuated pure shift spectra #include #include #include #include define list diff= define delay tauA define delay tauB define delay tauC "in0=inf1/2" "p2=p1*2"; 180 and 90 degree pulses ;ZanggerSterk delays "tauA=in0/2-p16-d16-50u" "tauB=in0-p16-d16-300u" "tauC=in0/2-p16-d16-350u-(dw*2)-(dw*2*cnst4)-de" ;Doneshot delays "cnst15=1+cnst14" ; 1 + alpha "cnst16=1-cnst14" ; 1 - alpha "DELTA=d20-4.0*p1-4*p30-5.0*d16-p19" ; diffusion encoding delay aqseq 312 1 ze 2 d1 pl1:f1 50u UNBLKGRAD ; Doneshot sequence 3 p19:gp2*-1.0 ; Spoiler gradient balancing pulse d16 p1 ph1 ; 1st 90 p30:gp1*cnst16*diff ; +(1 - alpha) d16 p2 ph2 ; First 180 p30:gp1*-1*cnst15*diff ; -(1 + alpha) d16 p1 ph3 ; 2nd 90 p30:gp1*2*cnst14*diff ; Lock refocusing pulse pulse, +(2*alpha) d16 p19:gp2 ; Spoiler gradient balancing pulse d16 DELTA ; diffusion encoding delay p30:gp1*2*cnst14*diff ; Lock refocusing pulse, +(2*alpha) d16 p1 ph4 ; 3rd 90 p30:gp1*cnst16*diff ; +(1 - alpha) d16 p2 ph5 ; 2nd 180 p30:gp1*-1*cnst15*diff ; -(1 + alpha) d16 ; Zangger-Sterk sequence 4 d0 tauA 50u p16:gp3*0.5 ; First gradient pulse d16 p2 ph6 ; Hard ZS 180 tauB p16:gp3*-0.5 d16 300u gron0 ; Slice selection gradient On p12:sp2:f1 ph7 ; Selective ZS 180 200u 100u groff ; Slice selection gradient Off p16:gp3*-1.0 d16 50u BLKGRAD tauC d0 5 go=2 ph31 d1 mc #0 to 2 F1QF(id0) F2QF(rd0, igrad diff) exit ;ph1 ; 1st 90 ;ph2 ; 1st 180 ;ph3 ; 2nd 90 ;ph4 ; 3rd 90 ;ph5 ; 2nd 180 ;ph6 ; Selective ZS 180 ;ph7 ; Hard ZS 180 ;ph31 ; Receiver ph31= ph1 + ph6*2+ ph7*2 ph1=0 2 0 2 1 3 1 3 0 2 0 2 1 3 1 3 0 2 0 2 1 3 1 3 0 2 0 2 1 3 1 3 0 2 0 2 1 3 1 3 0 2 0 2 1 3 1 3 0 2 0 2 1 3 1 3 0 2 0 2 1 3 1 3 ph2=0 ph3=0 ph4=0 ph5=0 ph6=0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 ph7=0 0 1 1 0 0 1 1 2 2 3 3 2 2 3 3 0 0 1 1 0 0 1 1 2 2 3 3 2 2 3 3 0 0 1 1 0 0 1 1 2 2 3 3 2 2 3 3 0 0 1 1 0 0 1 1 2 2 3 3 2 2 3 3 ph31=0 2 2 0 1 3 3 1 0 2 2 0 1 3 3 1 2 0 0 2 3 1 1 3 2 0 0 2 3 1 1 3 0 2 2 0 1 3 3 1 0 2 2 0 1 3 3 1 2 0 0 2 3 1 1 3 2 0 0 2 3 1 1 3 ;gp0 ;slice selective gradient ;gp1 ;diffusion encoding gradient ;gp2 ;spoiler gradien ;gp3 ;ZS CTP gradients ;p1 ;90 degree rf pulse width ;p2 ;180 degree rf pulse width ;p12 ;selective ZS pulse width ;p16 ;ZS CTP gradient width ;p19 ;spoiler gradient width ;p30 ;diffusion encoding gradient width ;pl1 ;f1 channel - power level for hard pulse (default) ;sp2 ;f1 channel - power level for soft pulse ;spoffs2 ;selective pulse offset (0 Hz) ;spnam2 ;file name for selective pulse ;d0 ;incremented delay ;d1 ;relaxation delay; 1-5 * T1 ;d16 ;gradient stabilisation delay ;d20 ;diffusion encoding delay ;td1 ;number of chunks to acquire ;cnst4 ;number of points to drop when collecting FI ;NS: ;DS: ;td1: number of experiments ; ;FnMODEs: QF ;Use AU program 'dosy' to calculate gradient levels ; ---Instructions--- ; ;pulse sequence = 90 - 0.5*t1 - tauA - 180H - tauB - 180ZS - tauC - 0.5*t1 - acquire ; ;(1) calibrate 90 degree hard pulse as normal ;(2) calibrate selective pulse using 'stdisp' ;(a) Calculate bandwidth ;this requires required bandwidth(Hz) and required rotation to produce a bandwidth (s) ;(b) Integrate shape ;this requires the bandwidth (s), required rotation and gives a change in power ; ;swh in F2 is an integer multiple of swh in F1 ;