;DISPEL (four stage) ; ; Pulse sequence for the suppression of one-bond 13C satellites in 1H spectra ; (with cw presaturation) ; ; Developed by: ; NMR Methodolgy Group ; University of Manchester ; ;Topspin 4.x ; ;$CLASS=HighRes ;$DIM=1D ;$TYPE= ;$SUBTYPE= ;$COMMENT= #include #include #include "acqt0=-p1*2/3.1416" "p2=p1*2" "d2=4050u" "d3=3200u" "d4=1100u" "d5=3950u" "d6=1560u" "d13=50u" "DELTA1=d3-d13-(p3/2)-d16-p16" "DELTA2=d2-d3-(p3/2)-(p2/2)" "DELTA3=d2-d3+d4-(p3/2)-(p2/2)-p16-d16" "DELTA4=d3-d4-(p3/2)-(p1/2)" "DELTA5=d5-p16-d16-(p1/2)-(p3/2)" "DELTA6=d2-d5-(p3/2)-(p2/2)" "DELTA7=d2-d5+d6-(p2/2)-(p3/2)-p16-d16" "DELTA8=d5-d6-(p3/2)" 1 ze 2 30m 20u BLKGRAD 30u pl9:f1 d1 2s cw:f1 ph29 4u do:f1 30u d13 pl1:f1 pl2:f2 3 (p1 ph1):f1 d13 UNBLKGRAD DELTA1 p16:gp1 d16 (p3 ph5):f2 DELTA2 (p2 ph2):f1 p16:gp1 d16 DELTA3 (p3 ph6):f2 DELTA4 (p1 ph3):f1 DELTA5 p16:gp2 d16 (p3 ph7):f2 DELTA6 (p2 ph4):f1 p16:gp2 d16 DELTA7 (p3 ph8):f2 DELTA8 (p1 ph9):f1 ;z-filter 10u gron0 pl0:f1 (p32:sp29 ph10):f1 20u groff d17 pl1:f1 p18:gp3 d17 (p1 ph11):f1 go=2 ph31 30m mc #0 to 2 F0(zd) 20u BLKGRAD exit ph1=0 2 ph2=0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 ph3=1 ph4=2 2 3 3 0 0 1 1 ph5=0 2 ph6=0 0 2 2 ph7=0 0 0 0 2 2 2 2 ph8=0 0 0 0 0 0 0 0 2 2 2 2 2 2 2 2 ph9=0 ph10=0 ph11=0 ph29=0 ph31=0 2 2 0 0 2 2 0 2 0 0 2 2 0 0 2 ;POWER LEVEL ;pl0 : 0W ;pl1 : f1 channel - power level for pulse (default) ;pl2 : f2 channel - power level for pulse (default) ;pl9 : f1 channel - power level for presaturation ;sp29: f1 channel - power level of adiabatic pulse of ZQF element ;PULSE SHAPE ;spnam29 : f1 channel - file name for the adiabatic shaped pulse using in ZQF [Crp60,20,20.10] ;PULSE DURATION ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p3 : f2 channel - 90 degree high power pulse ;p32: f1 channel - 180 degree shaped pulse (adiabatic) [20 msec] ;GRADIENT DURATION ;p16: duration of CTP gradients [1 msec] ;p18: homospoil gradient [1 msec] ;DELAY ;d1 : relaxation delay; 1-5 * T1 ;d13: delay for UNBLKGRAD/BLKGRAD ;d16: delay for gradient recovery [500usec] ;d17: delay for gradient recovery during ZQF [20msec] ;GRADIENT SHAPE ;gpnam1 : SMSQ10.100 ;gpnam2 : SMSQ10.100 ;gpnam3 : SMSQ10.100 ;GRADIENT STRENGTH ;gpz0 : ZQF gradient [3%] ;gpz1 : CTP gradient [43%] ;gpz2 : CTP gradient [37%] ;gpz3 : homospoil gradient [31%] ;OTHER ;ns: 1 * n, total number of scans: NS * TD0 ;ds: 2